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Directed mutagenesis of the transmembrane domain of the PsbL subunit of photosystem II inSynechocystissp. PCC 6803

โœ Scribed by Hao Luo; Julian J. Eaton-Rye


Book ID
106505294
Publisher
Springer
Year
2008
Tongue
English
Weight
587 KB
Volume
98
Category
Article
ISSN
0166-8595

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To identify amino acid residues of the D2 protein that are critical fo r functional photosystem II (PS II), sodium bisulfite was utilized for in vitro random mutagenesis of the psbDI gene from Synechocystis sp. PCC 6803. Sodium bisulfite reacts specifically with cytosine in single-stranded regions o

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In photosystem I (PSI) of oxygenic photosynthetic organisms thepsaC polypeptide, encoded by thepsaC gene, provides the ligands for two [4Fe-4S] clusters, FA and FB. Unlike other cyanobacteria, two different psaC genes have been reported in the cyanobacterium Synechocystis 6803, one (copy 1) with a d