To identify amino acid residues of the D2 protein that are critical fo r functional photosystem II (PS II), sodium bisulfite was utilized for in vitro random mutagenesis of the psbDI gene from Synechocystis sp. PCC 6803. Sodium bisulfite reacts specifically with cytosine in single-stranded regions o
โฆ LIBER โฆ
Engineered ectopic expression of thepsbAgene encoding the photosystem II D1 protein inSynechocystissp. PCC6803
โ Scribed by Madhavi Kommalapati; Hong Jin Hwang; Hong-Liang Wang; Robert L. Burnap
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 326 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0166-8595
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Random chemical mutagenesis of a specifi
โ
Svetlana Ermakova-Gerdes; Sergey Shestakov; Wim Vermaas
๐
Article
๐
1996
๐
Springer
๐
English
โ 890 KB
Photosystem II activity and turnover of
โ
Shlomit Tal; Nir Keren; Joseph Hirschberg; Itzhak Ohad
๐
Article
๐
1999
๐
Elsevier Science
๐
English
โ 646 KB
Site-directed psbA mutants at the tyrosine Y 112 position have been generated in Synechocystis PCC6803 cells. The mutation Y 112F does not affect photosystem II (PSII) activity as compared with control 4AIK cells. However, the Y112L mutant exhibits a photosynthetically impaired phenotype. PSII activ