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Directed evolution of a dextransucrase for increased constitutive activity and the synthesis of a highly branched dextran

✍ Scribed by Hee-Kyoung Kang; Eun-Seong Seo; John F. Robyt; Doman Kim

Book ID
113803678
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
232 KB
Volume
26
Category
Article
ISSN
1381-1177

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Directed evolution of an esterase for th
Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones
✍ Uwe T. Bornscheuer; Josef Altenbuchner; Hartmut H. Meyer πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 123 KB πŸ‘ 3 views

The directed evolution of an esterase from Pseudomonas fluorescens using the mutator strain Epicurian coli XL1-Red was investigated. Mutants were as- sayed for their ability to hydrolyze a sterically hindered 3-hydroxy ester, which can serve as a building block in the synthesis of epothilones. Scree