## Abstract We have shown previously that responses to lysophosphatidic acid (LPA) in __Xenopus__ oocytes exhibit pronounced rapid homologous desensitization mediated by Go family of G‐proteins (Itzhaki‐Van Ham et al., 2004, J Cell Physiol, 200: 125–133). The present study was aimed at examining th
Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes
✍ Scribed by Juan Martinez-Pinna; Iman S. Gurung; Martyn P. Mahaut-Smith; Andrés Morales
- Book ID
- 110116976
- Publisher
- Cambridge University Press
- Year
- 2010
- Tongue
- English
- Weight
- 412 KB
- Volume
- 588
- Category
- Article
- ISSN
- 0022-3751
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In Xenopus oocytes, both sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) activate Ca2+-dependent oscillatory Cl- currents by acting through membrane-bound receptors. External application of 50 microM S1P elicited a long-lasting oscillatory current that continued over 30 min from the be
## Abstract Native __Xenopus__ oocytes exhibit dose‐dependent depolarizing current responses to lysophosphatidic acid (LPA), with EC50 = 0.18 μM. Responses to LPA were subject to pronounced rapid desensitization. When oocytes were challenged with 5 nM LPA, the response was <10% of the maximal. Subs