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Direct transfer of whole genomes from bacteria to yeast

✍ Scribed by Karas, Bogumil J; Jablanovic, Jelena; Sun, Lijie; Ma, Li; Goldgof, Gregory M; Stam, Jason; Ramon, Adi; Manary, Micah J; Winzeler, Elizabeth A; Venter, J Craig; Weyman, Philip D; Gibson, Daniel G; Glass, John I; Hutchison, Clyde A; Smith, Hamilton O; Suzuki, Yo

Book ID
120154916
Publisher
Nature Publishing Group
Year
2013
Tongue
English
Weight
370 KB
Volume
10
Category
Article
ISSN
1552-4450

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Transfer of yeast artificial chromosomes
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✍ Clare Huxley; Andreas Gnirke 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 729 KB

Human DNA can he cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any chang