✦ LIBER ✦

Direct structural analysis of 2-aminoacridone derivatized oligosaccharides by high-performance liquid chromatography/mass spectrometric detection

✍ Scribed by Patrick Camilleri; David Tolson; Helen Birrell

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 140 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19980214)12:3<144::aid-rcm133>3.0.co;2-7

No coin nor oath required. For personal study only.

✦ Synopsis

In this preliminary study linear and branched oligosaccharides, derivatized with 2-aminoacridone, have been separated by reverse-phase high-performance liquid chromatography, and detected by positive-ion electrospray mass spectrometry. Tandem Mass spectra of the protonated carbohydrate analytes gave simple fragmentation patterns from which sequence information could be readily obtained.

📜 SIMILAR VOLUMES

Direct detection method of oligosacchari

Direct detection method of oligosaccharides by high-performance liquid chromatography with charged aerosol detection

✍ Shinsuke Inagaki; Jun Zhe Min; Toshimasa Toyo'oka 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB 👁 2 views

Structural analysis of chromophore-label

Structural analysis of chromophore-labeled disaccharides and oligosaccharides by electrospray ionization mass spectrometry and high-performance liquid chromatography/electrospray ionization mass spectrometry

✍ Li, D. T.; Her, G. R. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 296 KB 👁 2 views

Disaccharides and linear oligosaccharides were labeled with p-aminobenzoic ethyl ester (ABEE) chromophore and analyzed by negative ion electrospray ionization mass spectrometry (ESIMS). The formation of glycosylamines rather than reductive amination in the labeling reaction produced many characteris

Enantiomeric separation and detection by

Enantiomeric separation and detection by high-performance liquid chromatography-mass spectrometry of 2-arylpropionic acids derivatized with benzofurazan fluorescent reagents

✍ Tomofumi Santa; Jinli Luo; Chang-Kee Lim; Kuzuhiro Imai 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 121 KB 👁 2 views

The enantiomneric separation and the detection of 2-arylpropionic acids after derivatization with the fluorescent reagents with a benzofurazan structure, (S)-( + )-4-(N,N-dimethylaminosulphonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole ((S)-DBD-Apy), (R)-(-)-4-nitro-7-(3-aminopyrrolidin-1-yl)

Phenyl ring structures as stationary pha

Phenyl ring structures as stationary phases for the high performance liquid chromatography electrospray ionization mass spectrometric analysis of basic pharmaceuticals

✍ S. R. Needham; P. R. Brown; K. Duff 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 85 KB 👁 2 views

To produce a large mass spectral signal in the analysis of basic drugs by high performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS), a high percentage of organic solvent is needed in the mobile phase. With the commonly used reversed phase columns (C18, C8 and C4)

Analysis of Vero toxins 1 and 2 by high-

Analysis of Vero toxins 1 and 2 by high-performance liquid chromatography/electrospray ionization mass spectrometry

✍ Kondo, Fumio; Kobayashi, Shinichi; Matsumoto, Masakado; Yamada, Seiji; Saito, Ma 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB 👁 2 views

Analysis of Vero Toxins 1 and 2 by High-performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry During the past 10 years, disease outbreaks of hemorrhagic colitis (HC) and hemolyticÈuremic syndrome (HUS) in humans have been increasing worldwide owing to the ingestion of beef and

Characterization of derivatization of si

Characterization of derivatization of sialic acid with 2-aminoacridone and determination of sialic acid content in glycoproteins by capillary electrophoresis and high performance liquid chromatography - ion trap mass spectrometry

✍ Fa-Yun Che; Xiao-Xia Shao; Ke-Yi Wang; Qi-Chang Xia 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 187 KB 👁 2 views

A simple and highly sensitive capillary electrophoresis (CE) method for determining the content of N-acetylneuraminic acid (Neu5Ac) in glycoproteins was developed. Neu5Ac was derivatized with 2-aminoacridone (AMAC) by reductive amination, and the AMAC-Neu5Ac adduct could be readily separated from th