Direct somatic embryogenesis and plant r
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Xia Guang-min; Li Zhongyi; Guo Guang-qin; Chen Hui-min
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Article
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1992
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Springer
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English
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Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditio