The oxygen radical absorbance capacity (ORAC) assay has been used to quantify the antioxidative properties of phytonutrients in fruit and vegetable extracts. Using aqueous methanol extracts of tea and spinach as a model systems, separation of the components in the extracts by HPLC followed by semiau
Direct Obtention of Pure Compounds from Crude Plant Extracts by Preparative Liquid Chromatography
✍ Scribed by Kurt Hostettmann; Michael J. Pettei; Isao Kubo; Koji Nakanishi
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- German
- Weight
- 180 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0018-019X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
It has been possible to submit the crude hexane extract of the barks of a medicinal plant directly to preparative liquid chromatography. A two step solvent elution gave 6 peaks, four of which readily afforded crystalline material. The entire process required 2–3 hours whereas conventional chromatography required ca. 2 weeks.
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## Abstract A preparative high‐speed counter‐current chromatography method for isolation and purification of flavonoid compounds from __Oroxylum indicum__ was successfully established by using ionic liquids as the modifier of the two‐phase solvent system. Two flavonoid compounds including baicalein