𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Direct mitogenic effect of ionophore A23187 on isolated human T helper lymphocytes

✍ Scribed by Karl E. O. Åkerman; Leif C. Andersson

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
297 KB
Volume
14
Category
Article
ISSN
0014-2980

No coin nor oath required. For personal study only.

✦ Synopsis

The Ca2+ ionophore A23187 induces only a weak mitogenic response in cultures of unfractionated mononuclear leukocytes from human blood. When a comitogen, 12-O-tetradecanoyl-phorbol-13-acetate, which by itself is nonmitogenic, is added, a greatly increased cell proliferation is obtained. Highly purified T lymphocytes respond by proliferation to A23187 alone. Studies on functional subsets of T cells, fractionated by using the monoclonal antibodies OKT4/OKT8, revealed that A23187 is a strong mitogen for pure T helper cells (T4+). This suggests that increased cytosolic Ca2+ directly triggers a proliferative response in T helper cells with no apparent need for accessory cells.

📜 SIMILAR VOLUMES

Generation of Melan-A/MART-1-specific CD
Generation of Melan-A/MART-1-specific CD8+ cytotoxic T lymphocytes from human naive precursors: Helper effect requirement for efficient primary cytotoxic T lymphocyte induction in vitro
✍ Marina Ostankovitch; Frédérique-Anne Le Gal; Francine Connan; Dorine Chassin; Je 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 162 KB 👁 2 views

This study investigates the generation of primary melanoma cell-specific cytotoxic T lymphocytes (CTLs) in vitro. Induction of peptide-specific CTLs from unfractionated naive peripheral blood mononuclear cells from HLA-A2 healthy donors was assessed using 2 recently described 9-mer epitopes from the

A recombinant extracellular domain of th
A recombinant extracellular domain of the human interleukin 4 receptor inhibits the biological effects of interleukin 4 on T and B lymphocytes
✍ Pierre Garrone; Odile Djossou; Jean-Pierre Galizzi; Jacques Banchereau 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 486 KB

## Abstract Human interleukin 4 (IL 4) acts on various hematopoietic cell types through interaction with a specific cell surface receptor (IL 4R), whose cDNA has been cloned. We have produced a cDNA encoding a soluble form of the extracellular domain of the human IL 4R (sIL 4R) and describe here th

Effects of bacillus calmette-guerin on c
Effects of bacillus calmette-guerin on cytotoxic activities of peripheral blood lymphocytes against human T24 lined and freshly isolated autologous urinary bladder transitional carcinoma cells in patients with urinary bladder cancer
✍ Youichi Mizutani; Yoshinori Nio; Manabu Fukumoto; Osamu Yoshida 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 867 KB

The effects of bacillus Calmette-Guerin (BCG) on the cytotoxic activities of peripheral blood lymphocytes against human T24 lined and freshly separated autologous urinary bladder transitional carcinoma cells in patients with urinary bladder cancer were analyzed in a 12-hour chromium 51 ( 51Cr) relea

The effect of the interferon-γ-inducible
The effect of the interferon-γ-inducible processing machinery on the generation of a naturally tumor-associated human cytotoxic T lymphocyte epitope within a wild-type and mutant p53 sequence context
✍ Ulrike Kuckelkorn; Edite Antunes Ferreira; Ilse Drung; Ulrike Liewer; Peter-Mich 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 168 KB 👁 1 views

The human wild-type (wt) p53.264-272 peptide is a universal tumor antigen and recognized by HLA-A\*0201 (A2.1)-restricted CTL. Generation of this epitope by constitutive 20S proteasomes is prevented by a p53 R to H hotspot mutation at the C-terminal flanking residue 273. We report on the impact of t