Direct injection hplc analysis of some non-steroidal anti-inflammatory drugs on restricted access media columns

✍ Scribed by Ahsanul Haque; James T. Stewart

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 75 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199902)13:1<51::aid-bmc814>3.0.co;2-t

✦ Synopsis

Direct serum injection methods are described for the HPLC determination of selected non-steroidal anti-inflammatory drugs (NSAIDS) on restricted access media (RAM) columns, using either a Pinkerton (ISRP) or semi-permeable surface (SPS) column. Twenty microlitres of a filtered serum sample was injected directly onto each column. Calibration curves were prepared for ketoprofen (1-20 mg/mL), fenbufen (0.8-20 mg/mL), sulindac (0.8-20 mg/mL) and probenecid (1.5-40 mg/mL) on the SPS column, and nabumetone (1.5-40 mg/mL) and indomethacin (1.5-40 mg/mL) on the Pinkerton column. The percentage error and imprecision of the methods were found to be less than 10%. The inter-and intra-day variability for analytes were in the range of 0.15-10%. The limits of quantitation and detection were in the range of 800-1500 and 300-800 ng/mL, respectively, for the analytes studied. A hydrophobic shielded phase (Hisep) column was also investigated and was found to be generally too retentive (b29 min) for assay of these analytes.