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Direct hybridization of biotin-labeled probes in dried agarose gels

โœ Scribed by Nelder F. Gontijo; Julio Cesar C. Ribeiro; Sergio D.J. Pena

Book ID
113288950
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
183 KB
Volume
6
Category
Article
ISSN
0168-9525

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Direct hybridization of labeled DNA to D
Direct hybridization of labeled DNA to DNA in agarose gels
โœ M. Purrello; I. Balazs ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 989 KB

A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive pro