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Direct growth stimulation of normal human epithelial cells by mutant p53

✍ Scribed by Fiona S. Wyllie; Nick R. Lemoine; Claire M. Barton; Tim Dawson; Jane Bond; David Wynford-Thomas

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 559 KB
Volume: 7
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940070205

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We developed a high‐titer amphotropic retroviral vector that expresses mutant (Ala 143) human p53 to test directly the response of genetically normal human epithelial cells to p53 mutation. Contrary to our prediction, we found that in pancreatic epithelium (whose tumors display a high frequency of p53 mutation) but not in thyroid (whose tumors show an exceptionally low mutation frequency), expression of mutant p53 induced a dramatic, though self‐limiting, proliferative response. This result questions the assumption that p53 mutation is relevant only to the later stages of tumorigenesis.

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