✦ LIBER ✦

Effect of serum growth factors and phorbol ester on growth and survival of human thyroid epithelial cells expressing mutant ras

✍ Scribed by Jane Bond; Tim Dawson; Nick Lemoine; David Wynford-Thomas

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 776 KB
Volume: 5
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940050208

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We previously demonstrated a high incidence of ras mutation in thyroid follicular (epithelial) cell neoplasms and showed that expression of mutant ras is a potent mitogenic stimulus for normal human follicular cells in culture. Here we show that induction of cell proliferation in primary follicular cells by a mutant human Ha‐ras (val 12) expressed from a retroviral vector was absolutely dependent on the presence of serum growth factors. Induction of DNA synthesis showed partial dependence. Mutant ras‐induced growth was also inhibited by exposure to phorbol ester at concentrations sufficient to downregulate protein kinase C. More importantly, we observed an unexpected toxic effect of phorbol ester in this system that was specific to cells expressing mutant ras. This has potential significance both for elucidating the basic mechanism of ras action in epithelial cells and also as a pointer to a novel therapeutic strategy. © 1992 wiley‐list, Inc.

📜 SIMILAR VOLUMES

Effects of tyrosine kinase inhibitor, er

Effects of tyrosine kinase inhibitor, erbstatin, on cell growth and growth-factor/receptor gene expression in human gastric carcinoma cells

✍ Naoki Takekura; Wataru Yasui; Eikai Kyo; Kazuhiro Yoshida; Takashi Kameda; Yasuh 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 571 KB

The effect of tyrosine kinase inhibitor, erbstatin, on cell growth and mRNA expression of growth-factor/receptor system was examined in 6 human gastric-carcinoma cell lines. Erbstatin inhibited both EGF-induced and serum-stimulated cell growth of all 6 cell lines (TMK-I, MKN-I, -7, -28, -45, -74) in

Effects of growth factors, hormones, bac

Effects of growth factors, hormones, bacterial lipopolysaccharides, and lipotechoic acids on the clonal growth of normal ureteral epithelial cells in serum-free culture

✍ John J. Wille; Jong Park; Ada Elgavish 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 775 KB

## Abstract In vitro tissue culture techniques were employed to study the effects of bacterial endotoxins on the growth of normal epithelial cells from the human ureter (NHU). Primary cultures of NHU cells were initiated from explant outgrowth cultures of human ureteral tissue and cultured on colla

Modulation by staurosporine of phorbol-e

Modulation by staurosporine of phorbol-ester-induced effects on growth and protein kinase C localization in a549 human lung-carcinoma cells

✍ Tracey D. Bradshaw; Andreas Gescher; George R. Pettit 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 French ⚖ 711 KB

I 2-0-tetradecanoylphorbol-I 3-acetate (TPA) and bryostatin I are activators of protein kinase C (PKC). TPA is a potent inhibitor of the growth of A549 cells, while bryostatin I exerts a weak antiproliferative effect upon this cell line. We tested the hypothesis that the PKC inhibitor staurosporine

EFFECTS OF TRANSFORMING GROWTH FACTOR-β1

EFFECTS OF TRANSFORMING GROWTH FACTOR-β1 AND FIBRONECTIN ON SPARC EXPRESSION IN CULTURES OF HUMAN PERIODONTAL LIGAMENT CELLS

✍ Tsuyoshi Fujita; Hideki Shiba; Masatoshi Sakata; Yuushi Uchida; Tetsuji Ogawa; H 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 350 KB

Response of normal and oncogene-transfor

Response of normal and oncogene-transformed human mammary epithelial cells to transforming growth factor β1 (TGF-β1): Lack of growth-inhibitory effect on cells expressing the simian virus 40 large-t antigen

✍ Fulvio Basolo; Lisa Fiore; Fortunato Ciardiello; Simonetta Calvo; Gabriella Font 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 825 KB

The relationship between the expression of selected oncogenes having different modes of action and the loss of the capacity t o respond in vitro to transforming growth factor+ I (TGF-P I) was analyzed in human mammary epithelial cells (MEC). Primary MEC cultures from healthy donors and the spontaneo

Effects of inhibitors of signal transduc

Effects of inhibitors of signal transduction pathways on transforming growth factor B1 and osteogenic protein-1-induced insulinlike growth factor binding protein-3 expression in human bone cells

✍ Narasimhan Srinivasan; David J. Baylink; Kuber Sampath; Subburaman Mohan 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 202 KB 👁 2 views

Signal transduction initiated by TGFb1 and OP-1 was studied in MG63 human osteosarcoma cells and in normal human bone cells (HBCs) in the presence of inhibitors of signal transduction events, using insulinlike growth factor binding protein-3 (IGFBP-3) production as an end point. Treatment of serum-f