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Direct gene transfer to protoplasts ofArabidopsis thaliana

✍ Scribed by Hans Karesch; Roland Bilang; Ortrun Mittelsten Scheid; Ingo Potrykus

Book ID
104674974
Publisher
Springer
Year
1991
Tongue
English
Weight
991 KB
Volume
9
Category
Article
ISSN
0721-7714

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✦ Synopsis

We performed a series of direct gene transfer experiments with protoplasts of Arabidopsis thaliana ecotype ZΓΌrich. An average of more than 100 transformants were selected per 10(6)6 treated protoplasts. Stable transformation was confirmed by integration of the marker gene into high molecular weight DNA and by its genetic transmission to subsequent offspring generations.

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