Transgenic Rice Plants After Direct Gene Transfer into Protoplasts
β Scribed by Toriyama, Kinya ;Arimoto, Youichi ;Uchimiya, Hirofumi ;Hinata, Kokichi
- Book ID
- 109907625
- Publisher
- Nature Publishing Group
- Year
- 1988
- Tongue
- English
- Weight
- 488 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1087-0156
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π SIMILAR VOLUMES
A novel procedure employing mild sonication for transformation of plant protoplasts is described. Transient expression of a chloramphenicol acetyltransferase (CAT) gene in protoplasts of sugar beet (Beta vulgaris L.) and tobacco (Nicotiana tabacum L.) was obtained by a brief exposure of the protopla
In P. hybrida and B. nigra an enhancement of transformation rates (direct gene transfer) of about six to seven-fold was obtained after irradiation of protoplasts with 12.5 Gy (X-ray). The effect of protoplast irradiation was similar in experiments where protoplasts were irradiated 1h before transfor
The efficiency of electroporation of sugar beet (Beta vulgaris L.) and tobacco (Nicotiana tabacum L.) protoplasts by alternating, rectangular and exponentially decaying pulses was studied by assaying transient expression of an introduced gene for chloramphenicol acetyltransferase. A simple device fo