Direct detection of nif-gene sequences of Enterobacter agglomerans in soil
✍ Scribed by Sonja Selenska; Walter Klingmüller
- Book ID
- 109317115
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 178 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The nucleotide sequence of a plasmid-borne 3.9 kb XhoI-SmaI fragment comprising the 3'-region of the nifM gene, the nifL and nifA genes and the 5'region of nifB gene of Enterobacter a991omerans was determined. The genes were identified by their homology to the corresponding n/f genes of Klebsiella p
A small plasmid containing the entire nif gene cluster of Enterobacter agglomerans 333 as an excisable cassette has been constructed, using pACYC177 as a vector. Two cosmid clones taken from a gene library of E. agglomerans plasmid pEA3 were used as a source of nif genes. A SmaI fragment of peaMS2-2
The nifQ gene, involved in early stages of iron-molybdenum cofactor (FeMo-co) biosynthesis, was identified downstream of the nifB and nifF genes of Enterobacter agglomerans. This gene was cloned and its nucleotide sequence determined. The amino acid sequence, as deduced from the nucleotide sequence,