Location of nitrogen fixation (nif) genes on indigenous plasmids of Enterobacter agglomerans
✍ Scribed by Singh, Mahavir ;Kleeberger, A. ;Klingmüller, W.
- Book ID
- 104725490
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 610 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0026-8925
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A small plasmid containing the entire nif gene cluster of Enterobacter agglomerans 333 as an excisable cassette has been constructed, using pACYC177 as a vector. Two cosmid clones taken from a gene library of E. agglomerans plasmid pEA3 were used as a source of nif genes. A SmaI fragment of peaMS2-2
The nucleotide sequence of a plasmid-borne 3.9 kb XhoI-SmaI fragment comprising the 3'-region of the nifM gene, the nifL and nifA genes and the 5'region of nifB gene of Enterobacter a991omerans was determined. The genes were identified by their homology to the corresponding n/f genes of Klebsiella p