Direct demonstration that the AγT globin gene is linked to the 4 bp promoter deletion in the βA chromosome of sickle cell traits

Polymerase chain reaction amplification

Polymerase chain reaction amplification applied to the direct detection of a 4 bp deletion in the promoter region of the Aγ gene

✍ Laura Manca; Elena Cocco; Bruno Masala; Dr John G. Gilman 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB 👁 2 views

We report here the identification of a 4 bp deletion in the 'yT globln gene promoter by means of Fnu4HI digestion of DNA amplified by polymerase chain reaction (PCR). This deietlon has been prevlously associated with haplotype II p-thalassemia in Sardinia. This simple, non-radioactive procedure shou