Direct and continuous spectrophotometric assay of phosphomonoesterases
โ Scribed by B.H.J. Hofstee
- Book ID
- 118840423
- Publisher
- Elsevier Science
- Year
- 1954
- Tongue
- English
- Weight
- 366 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0003-9861
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A continuous spectrophotometric assay for pantetheinase determination using S-pantetheine-3-pyruvate as substrate is described. The enzymatic hydrolysis of this new substrate leads to the formation of S-cysteamine-3-pyruvate, which cyclizes in a non-rate-limiting step to give 2H-1 +thiazin-5,6dihydr