A direct UV-VIS spectrophotometric assay has been developed for peptide deformylase. This assay employs a novel class of peptide mimetics as deformylase substrates which, upon enzymatic removal of the Nterminal formyl group, rapidly release free thiols. The released thiols are quantitated using Ellm
Continuous Spectrophotometric Assay of Peptide Deformylase
โ Scribed by Yaoming Wei; Dehua Pei
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 137 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0003-2697
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