✦ LIBER ✦

Direct Analysis of Odors Emitted by Freshly Cut Allium Using Combined High-performance Liquid Chromatography and Mass Spectrometry

✍ Scribed by S. Ferary; E. Thibout; J. Auger

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 562 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199608)10:11<1327::aid-rcm560>3.0.co;2-3

No coin nor oath required. For personal study only.

✦ Synopsis

Allium odors were re-examined to determine whether or not they contain degradation compounds of thiosulfinates. Garlic (Allium sativum), leek (Allium porrum) and onion (Allium cepa) were investigated using cryotrapping and direct injection of aqueous solutions of the samples into a high-performance liquid chromatograph (HPLC), with a diode-array detector, coupled to a maw spectrometer (MS). Various commercial systems which couple HPLC to tandem MS, but using different ionization systems, were compared. Best results were obtained with atmospheric pressure chemical ionization although degradation of certain thiosulfinates was observed. Depending on the HPLC/MS interface, thiosulfinates formed dimers in variable quantity and this reaction could be used to differentiate isomeric 1-propenyl and 2-propenyl thiosulfinates. Some unresolved isomers were identified by their fragment ions. No disulfides or other rearrangement products were observed and it was concluded that Allium odors contain only thiopropanal S-oxide and thiosulfinates as sulfur volatiles.

📜 SIMILAR VOLUMES

Analysis of native neutral glycosphingol

Analysis of native neutral glycosphingolipids by combined high performance liquid chromatography/mass spectrometry

✍ James E. Evans; Robert H. McCluer 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 535 KB

Analysis of 9-fluorenylmethyloxycarbonyl

Analysis of 9-fluorenylmethyloxycarbonyl derivatives of catecholamines by high performance liquid chromatography, liquid chromatography/mass spectrometry and tandem mass spectrometry

✍ Su Chen; Qing Li; Paul M. Carvey; Kawan Li 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 142 KB 👁 3 views

9-Fluorenylmethyloxycarbonyl (FMOC) derivatives of catecholamines, including dopamine (DA; 3,4-dihydroxyphenethylamine), norepinephrine (NE; 2-amino-1-(3,4-dihydroxyphenyl)ethanol) and epinephrine (EPI; 1-phenyl-1-hydroxy-2-methylaminopropane) as well as 3, 4-dihydroxybenzylamine (DHBA) have been an

Isolation and Characterization of Conope

Isolation and Characterization of Conopeptides by High-performance Liquid Chromatography Combined with Mass Spectrometry and Tandem Mass Spectrometry

✍ Alun Jones; Jon-Paul Bingham; John Gehrmann; Trudy Bond; Marion Loughnan; Ann At 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 479 KB 👁 2 views

The sensitivity of mass spectrometry combined with the separatory power of high-performance liquid chromatography was used to investigate the venom of individual cone shells. This analysis has revealed that cone venoms contain a complex mixture of peptides which vary quantitatively and qualitatively

Rapid analysis of tetracycline antibioti

Rapid analysis of tetracycline antibiotics by combined solid phase microextraction/high performance liquid chromatography/mass spectrometry

✍ Chris M. Lock; Luke Chen; Dietrich A. Volmer 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 177 KB 👁 2 views

The technique of solid phase microextraction (SPME) combined on-line with high performance liquid chromatography/mass spectrometry (HPLC/MS) has been applied to the analysis of seven tetracycline analogues. Rapid baseline separation was achieved in under 5 min using a short 3 microm RP-18e cartridge

Direct measurement of urinary testostero

Direct measurement of urinary testosterone and epitestosterone conjugates using high-performance liquid chromatography/tandem mass spectrometry

✍ Borts, David J.; Bowers, Larry D. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 163 KB 👁 2 views

## Measurement of the ratio of testosterone (T) and epitestosterone (E) in urine has been used as an indication of 'natural' steroid supplementation for a decade. The direct measurement of the glucuronide and sulfate conjugates of testosterone and epitestosterone by high-performance liquid chromato

Direct injection versus liquid-liquid ex

Direct injection versus liquid-liquid extraction for plasma sample analysis by high performance liquid chromatography with tandem mass spectrometry

✍ Mohammed Jemal; Mike Huang; Xiaohui Jiang; Yan Mao; Mark L. Powell 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB 👁 3 views

Direct injection versus liquid-liquid extraction for post-dose human plasma sample analysis by high performance liquid chromatography with tandem mass spectrometry (LC/MS/MS) have been studied using a drug candidate compound. For the direct-injection method, an Oasis 1 HLB column (1 Â 50 mm, 30 mm)