The informativeness of locus D11S29 as a genetic marker was improved by the identification of a highly polymorphic (GT)n repeat within the locus. Ten alleles were identified in parents of 40 Centre d'Etude du Polymorphisme Humain families and the polymorphism information content (PIC) was 0.77. The
Dinucleotide repeat polymorphism at the D5S99 locus on chromosome 5q33–34
✍ Scribed by Ilse M. Groenewald; Louise Warnich; Andries E. Retief
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 834 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
We report a highly polymorphic, sequencetagged microsatellite site (STMS) at the D5S99 locus that was previously identified by a less informative restriction fragment length polymorphism (RFLP). This marker, which was also localized to the physical map of chromosome 5q by fluorescent in situ hybridization (FISH), should assist in the precision mapping of genes in the area 5q33-34.
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Loss of heterozygosity at the TP53 locus occurs frequently in many types of cancer and requires polymorphic markers for detection. Several polymorphisms at the TP53 locus have been described previously, and polymerase chain reaction (PCR)based assays have been developed to detect these polymorphisms