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Diffraction imaging of spheres and melanoma cells with a microscope objective

✍ Scribed by Kenneth M. Jacobs; Li V. Yang; Junhua Ding; Andrew E. Ekpenyong; Reid Castellone; Jun Q. Lu; Xin-Hua Hu

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
314 KB
Volume
2
Category
Article
ISSN
1864-063X

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✦ Synopsis

Abstract

Diffraction imaging of polystyrene spheres and B16F10 mouse melanoma cells embedded in gel has been investigated with a microscope objective. The diffraction images acquired with the objective from a sphere have been shown to be comparable to the Mie theory based projection images of the scattered light if the objective is translated to defocused positions towards the sphere. Using a confocal imaging based method to reconstruct and analyze the 3D structure, we demonstrated that genetic modifications in these cells can induce morphological changes and the modified cells can be used as an experimental model for study of the correlation between 3D morphology features and diffraction image data. (Β© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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