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Differentiation of regioisomers at 15N natural abundance using gradient-enhanced 1H/15N HMBC NMR spectroscopy

โœ Scribed by Patricia A. McDonnell; A. Diane Gauthier; Michael P. Ferro

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
195 KB
Volume
36
Category
Article
ISSN
0749-1581

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โœฆ Synopsis

The 1H/15N HMBC experiment at 15N natural abundance using pulsed รeld gradients is a useful tool for chemical shift assignment and structure elucidation. This experiment assisted in the identiรcation of regioisomers that could not be distinguished with conventional 1H/1H nuclear Overhauser or 1H/13C correlation NMR experiments. The natural abundance 15N gradient-enhanced HMBC experiment was easily implemented, provided high-quality spectra and readily distinguished nitrogen-containing regioisomers. This experiment can aid in structure elucidation when traditional 1H and 13C experiments fail to provide unique structural assignment. 1998 ( John Wiley & Sons, Ltd.

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