𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Differential utilization of the SV40 enhancer and early promoter in various human lung carcinoma cell lines

✍ Scribed by Yuk-Chor Wong; Samuel D. Bernal

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
643 KB
Volume
45
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Cell lines derived from the major morphological types of human lung cancer were tested for their ability to utilize the SV40 enhancerlearly promoter. These cell lines were transfected with a recombinant plasmid containing a reporter gene, coding for chloramphenicol acetyltransferase (CAT), under the control of the SV40 enhancerlearly promoter. The transfected cells were then assayed for CAT activity. Nonsmall-cell carcinomas, especially squamous carcinomas, were found to be 2-3 orders of magnitude more efficient in utilizing the SV40 enhancerlearly promoter than small-cell carcinomas. The presence of different SV40 enhancerlearly promoter specific DNA-binding nuclear factors in squamous and small-cell carcinomas was demonstrated in gel mobility shift experiments. These observations seem to suggest that the set of transcriptional regulatory factors associated with squamous carcinomas may be distinct from that associated with small-cell carcinomas.

πŸ“œ SIMILAR VOLUMES

Characterization of the differential pro
Characterization of the differential protein expression associated with thermoresistance in human gastric carcinoma cell lines
✍ Pranav Sinha; Julia Poland; Martina SchnΓΆlzer; Julio E. Celis; Hermann Lage πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 301 KB πŸ‘ 2 views

## Characterization of the differential protein expression associated with thermoresistance in human gastric carcinoma cell lines Resistance to chemotherapeutic agents is one of the major problems faced during palliative therapy of tumor cells. Thus, chemotherapy is frequently combined with other

Intron/Exon organization and polymorphis
Intron/Exon organization and polymorphisms of the PLK3/PRK gene in human lung carcinoma cell lines
✍ Jonathan Wiest; Adam M. Clark; Wei Dai πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 127 KB

## Abstract __PLK3/PRK__, a conserved polo family protein serine/threonine kinase, plays a significant role at the onset of mitosis and mitotic progression. Recently, __PLK3/PRK__ has been shown to induce apoptosis when overexpressed in cell lines and is also implicated in cell proliferation and tu