Electrospray ionization coupled to a quadrupole ion trap mass spectrometer is used to differentiate between the isobaric amino acids lysine and glutamine in sequence analysis of peptides. Collision-induced dissociation is used for fragmentation. Several isobaric peptides with one or more lysines or
โฆ LIBER โฆ
Differential sequencing with mass spectrometry
โ Scribed by Joel H. Graber; Cassandra L. Smith; Charles R. Cantor
- Book ID
- 114342385
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 102 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1050-3862
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Key steps in a proposed automated system for polypeptide sequencing utilizing a liquid chromatograph mass spectrometer computer system have been tested with mixtures containing up to six model oligopeptides. At the low nanomole level it was possible to obtain complete sequence information for all co