Differential Replication of Plasmids during Stringent and Relaxed Response of Escherichia coli
✍ Scribed by Anna Herman; Alicja Wȩgrzyn; Grzegorz Wȩgrzyn
- Book ID
- 112263211
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 254 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0147-619X
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Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+:CP78; relA:CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated o
Replication of mini-plasmids derived from bacteriophage P1 and naturally existing plasmids F, R1, R6K and RK2 in otherwise isogenic relA+ and reLk Escherichia coli strains during amino acid starvation and limitation was investigated. Since it was previously demonstrated that inhibition of DNA synthe