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Differential quantitative determination of acetyl-CoA and acetyl-dephospho-CoA

✍ Scribed by Gerd Kloster; Hermann Eggerer

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
383 KB
Volume
65
Category
Article
ISSN
0003-2697

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✦ Synopsis

A fast and reliable enzymic method is presented for differential quantitative determination of acetyl-CoA and acetyl-dephospho-Cot either present in mixtures or separately. The sum of both compounds is determined by the assay with citrate synthase (EC 4.1.3.7), as known. Conditions are given under which acetyl-CoA but not acetyl-dephospho-CoA, present in the same solution, reacts with glyoxylate on malate synthase (EC 4.1.3.2). Thus, acetyl-CoA and acetyldephospho-CoA are easily determined quantitatively by use of the two enzymic assays.

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