Determination of pyruvate dehydrogenase and acetyl-CoA synthetase activities using citrate synthase

Measurement of ATP citrate-lyase and ace

Measurement of ATP citrate-lyase and acetyl-CoA synthetase activity using arylamine acetyltransferase

✍ Georg Hoffmann; Ludwig Weiss; Otto H. Wieland 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 456 KB

Methods are described for the assay of ATP citrate (pro-3S)-lyase (EC 4.1.3.8) and acetyl-CoA synthetase (EC 6.2.1.1) activity in crude tissue extracts. Arylamine acetyltransferase (EC 2.3.1.5) and p-nitroaniline serve as indicator system in these tests. The methods have several advantages with resp