Differential proliferative response of cultured fetal and regenerating hepatocytes to growth factors and hormones
โ Scribed by Carmen de Juan; Manuel Benito; Alberto Alvarez; Isabel Fabregat
- Book ID
- 115892688
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 874 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0014-4827
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Sustained production of plasma proteins, notably albumin, is a reliable indicator of the differentiated state ot hepatocytes. In this work, we have developed a fetal hepatocyte culture system where studying the regulation of albumin expression in proliferating liver cells. Our results show that unde
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## Abstract The culture of fetal hepatocytes for 64 h in medium supplemented with 5 mM glucose, T3, insulin, and dexamethasone resulted in the coordinate precocious expression of malic enzyme mRNA, protein, and specific activity. T3 was the main inducer; meanwhile, insulin exerted a small synergist