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Regulation of albumin expression in fetal rat hepatocytes cultured under proliferative conditions: Role of epidermal growth factor and hormones

✍ Scribed by Carmen De Juan; Manuel Benito; Isabel Fabregat

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 891 KB
Volume: 152
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041520113

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✦ Synopsis

Sustained production of plasma proteins, notably albumin, is a reliable indicator of the differentiated state ot hepatocytes. In this work, we have developed a fetal hepatocyte culture system where studying the regulation of albumin expression in proliferating liver cells. Our results show that under proliferative conditions (i.e., in the presence of EGF) fetal hepatocytes maintain albumin production above control quiescent non-treated cells. Glucagon and noradrenaline have no effect on the prolifcration induced by EGF in cultured fetal hepatocytcs; however, they act synergistically with this growth factor, increasing intracellular albumin levels. The maximum response is obtained by treatment of cells with EGF and noradrenaline. The stimulatory noradrenergic effect is mimicked by agents that increase cyclic AMP levels (forskolin plus IBMX). However, vasopressin or phorbol esters have no effect on albumin production, neither alone nor in combination with EGF. Dexamethasone, which does not alter the proliferative induction of EGF, increases albumin content. This effect is independent of the proliferative status of the cells and is not enhanced by glucagon, noradrenaline, or cyclic AMP increasing agents. The hormonal changes observed in albumin production partially correlate with changes in mRNA levels. This is the first time that cyclic AMP increasing agents are shown to act synergistically with ECF, increasing the expression of this liver specific gene. o 1992 WiIey-Li\s, Inr.

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