Differential precipitation with concanavalin a as a method for the purification of glycoproteins: Human alkaline phosphatase

A method for the determination of glucos

A method for the determination of glucose-6-phosphatase activity in rat liver with [U-14C]glucose 6-phosphate as substrate

✍ S.A. Kitcher; K. Siddle; J.P. Luzio 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 414 KB

A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the u