Differential expression of cell adhesion molecules in variants of K1735 melanoma cells differing in metastatic capacity

✍ Scribed by Dorte Linnemann; Avraham Raz; Elisabeth Bock

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 515 KB
• Volume: 43
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910430428

✦ Synopsis

We have investigated the expression of 2 neural-cell adhesion molecules, NCAM and LI, in K1735-CI16 and -MI melanoma cells which differ qualitatively in their metastatic potential, i.e., M l cells are metastatic whereas CI 16 cells are not. We have found that NCAM in C116 cells are expressed as 2 quantitatively major glycosylated polypeptides with Mr of 145,000 and 120,000 and a minor 190,000 Mr polypeptide, whereas M I cells expressed NCAM as 3 glycosylated polypeptides with Mr of 200,000, I40,OOO and 120.000. The amount of NCAM in MI cells constituted only 60% of the amount observed in C116 cells. In C116 cells, the 145,000 and 120,000 Mr NCAM polypeptides were sulphated whereas NCAM did not appear to be sulphated in M I cells. No phosphorylation of NCAM in the 2 cell lines was observed. L I was expressed as a phosphorylated glycoprotein with Mr of 210,000 in M I cells whereas no L I expression was observed in C116 cells. L I was not sulphated in MI cells.