Method for Simultaneous Detection of Pro
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H. Fukazawa; P.M. Li; S. Mizuno; Y. Uehara
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Article
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1993
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Elsevier Science
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English
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We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of \(\mathbf{v}-8\) src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and \(\left[\gamma-{ }^{32} \mathbf{P}\right] \mathrm{ATP}