Different Effects of Some Isoquinoline Alkaloids from Argemone mexicana on Electrically Induced Contractions of Isolated Guinea-pig Ileum

✍ Scribed by Sonia Piacente; Anna Capasso; Nunziatina De Tommasi; Cumanda Jativa; Cosimo Pizza; Ludovico Sorrentino

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 124 KB
• Volume: 11
• Category: Article
• ISSN: 0951-418X
• DOI: 10.1002/(sici)1099-1573(199703)11:2<155::aid-ptr48>3.0.co;2-9

✦ Synopsis

The present study examines the effects of the extracts [petroleum ether, CHCl 3 , CHCl 3 /MeOH (9:1) and MeOH], partially purified fractions and pure compounds from Argemone mexicana on the electrically induced contractions of the isolated guinea-pig ileum (E.C.I.).

The results of the experiments indicate that CHCl 3 /MeOH (9:1) and MeOH extracts, tested at a concentration of 400, 200 and 100 g/mL, dose-dependently reduced the guinea-pig ileum contractions, whereas the petroleum ether and CHCl 3 extracts did not affect it. Furthermore, the partially purified fractions II-V from the MeOH extract, each tested at concentrations of 200, 100 and 50 g/mL also inhibited E.C.I. Finally the pure compounds 1-2 (5ϫ 10 Ϫ 6 -1ϫ 10 Ϫ 5 -5ϫ 10 Ϫ 5 M) isolated and purified from the above fractions significantly reduced, in a dose dependent manner, the electrical contractions of the ileum, whereas compound 3 (5ϫ 10 Ϫ 6 -1ϫ 10 Ϫ 5 ϫ 10 Ϫ 5 M) increased them.

Since the active compounds 1-3 were respectively identified as protopine, allocryptopine and berberine by NMR, the observed effects could be related mainly to these compounds.