Constituents ofCroton menthodorus and their effects on electrically induced contractions of the guinea-pig isolated ileum

✍ Scribed by Anna Capasso; Sonia Piacente; Nunziatina De Tommasi; Massimo Ragucci; Cosimo Pizza

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 84 KB
• Volume: 14
• Category: Article
• ISSN: 0951-418X
• DOI: 10.1002/(sici)1099-1573(200005)14:3<156::aid-ptr630>3.0.co;2-v

✦ Synopsis

The present study examines the effects of the extracts [petroleum ether, CHCl 3 , CHCl 3 MeOH (9:1) and MeOH], partially purified fractions and pure compounds from Croton menthodorus on the electrically induced contractions of the isolated guinea-pig ileum (ECI).

The results of the experiments indicate that CHCl 3 /MeOH (9:1) and MeOH extracts, tested at concentrations of 100, 50 and 25 mg/mL, dose-dependently reduced the guinea-pig ileum contractions, whereas petroleum ether and CHCl 3 extracts did not affect it. Furthermore, the partially purified fractions III-VI from the CHCl 3 /MeOH extract, each tested at concentrations of 100, 50 and 25 mg/mL also inhibited ECI. Finally, pure compound 1 (6 Â 10 À6 , 3 Â 10 À6 , 1 Â 10 À6 M) isolated and purified from the most active fraction III significantly reduced, in a dose-dependent manner, the electrical contractions of the ileum.

Compound 1 was identified by NMR and EI-MS data as the morphinandien-7-one, O-methylflavinantine.