## Abstract Caffeine has been extensively used as a probe to measure CYP1A2 activity in humans with caffeine clearance or the paraxanthine (major metabolite of caffeine) to caffeine concentration ratio being regarded as the preferred metric. A simple reverse‐phased C~18~ HPLC assay using ethyl acet
✦ LIBER ✦
Differences in caffeine and paraxanthine metabolism between human and murine CYP1A2
✍ Scribed by Andreas Labedzki; Jeroen Buters; Wafaâ Jabrane; Uwe Fuhr
- Book ID
- 113993074
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 233 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0006-2952
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In this study the validation of a reversed-phase high-performance liquid chromatography (HPLC) method, with UVdetection, for both caffeine and paraxanthine in human serum is described. This method is feasible for cytochrome P450 1A2 (CYP1A2) phenotyping, according to the results of a pilot study. Wi
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