Dialysis of small samples in agarose gels
β Scribed by David Freifelder; Marc Better
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 240 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Reduction of the salt concentration of small volumes (0.1 ml or less) of solutions of macromolecules can be accomplished by placing the solution in a small well in an agarose gel. This technique has been especially useful in preparation of samples for electron microscopy.
π SIMILAR VOLUMES
A simple equilibrium dialysis cell may be quickly prepared from common, inexpensive microcentrifuge tubes. The resulting cell is easy to use and precise enough for quantitative dialysis studies of small samples (<SO ~1). In addition, by using a portion of the cell, exchange dialysis of small samples