Diagnosis of a deletion of steroid sulfatase by polymerase chain reaction and high-performance liquid chromatography
β Scribed by Teruo Sugawara; Masanori Iwaki; Seiichiro Fujimoto
- Book ID
- 117075744
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 323 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0009-8981
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conditions, but there existed a linear relationship between the molar ratio of input targets and the molar ratio of their corresponding output products. Using this mathematical approach, we have achieved very good accuracy (recovery %) of 100 + 16% and within-run imprecision (CV) of 12% for a quanti
Alpha-thalassemia of Southeast Asian deletion (--SEA/) is very common in Southeast Asia. Homozygosity of this genotype is the major cause of Hb Bart's hydrops fetalis in Taiwan. With polymerase chain reaction using three oligonucleotide primers bridging the common deletion breakpoint, a DNA fragment