Diadenosine 5′,5‴-P1,P4-tetraphosphate (Ap4A): Its role in cellular metabolism
✍ Scribed by Paul Zamecnik
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 884 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0003-2697
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A simple method for measuring the cellular content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cultured mammalian cells is described. Ap4A was rapidly extracted by dissolving cell monolayers using 0.1 N NaOH. It was separated from the bulk of cellular components in a single step by selecti
Diadenosine 5',5"'-P,,P,-tetraphosphate (Ap,A), a n intracellular regulatory nucleotide, has been found to react with the antitumor drug cw-diamminedichloroplatinum(I1J and its aqua derivative to form a single complex. This complex has been purified by highperformance liquid chromatography and chara