Structure determination of the adduct formed between the signal nucleotide diadenosine 5′, 5‴-P1, P4-tetraphosphate (Ap4A) and cis-[Pt(NH3)2Cl2]
✍ Scribed by Evelyne Segal; Jean-Pierre Girault; Gabriel Muzard; Geneviève Chottard; Jean-Claude Chottard; Jean-Bernard Le Pecq
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1984
- Tongue
- English
- Weight
- 625 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Diadenosine 5',5"'-P,,P,-tetraphosphate (Ap,A), a n intracellular regulatory nucleotide, has been found to react with the antitumor drug cw-diamminedichloroplatinum(I1J and its aqua derivative to form a single complex. This complex has been purified by highperformance liquid chromatography and characterized by 'H-nmr and CD spectroscopy. In this complex, Ap,A takes a very particular conformation. It is a n N7-N7 chelate of the metal with the two adenines in a head-to-head arrangement and a n anti-anti conformation of the adenosines. Platinum chelation leads to a large decrease of the Ap,A conformational flexibility.