Diabetic Nephropathy: Methods and Protocols

✍ Scribed by Luigi Gnudi, David A. Long

• Publisher: Springer US;Humana
• Year: 2020
• Tongue: English
• Leaves: 337
• Series: Methods in Molecular Biology 2067
• Edition: 1st ed. 2020
• Category: Library

✦ Synopsis

This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and to develop treatments for this condition.

✦ Table of Contents

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
Diabetic Nephropathy: An Overview (Manpreet K. Sagoo, Luigi Gnudi)....Pages 3-7
Front Matter ....Pages 9-9
Modeling Podocyte Biology Using Drosophila Nephrocytes (Paul S. Hartley, Richard J. Coward)....Pages 11-24
A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros (Maria Kolatsi-Joannou, Daniel Osborn)....Pages 25-39
Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model (Carolynn Cairns, Bryan Conway)....Pages 41-52
Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy (Elisavet Vasilopoulou)....Pages 53-59
Front Matter ....Pages 61-61
Histological Examination of the Diabetic Kidney (Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, Gianfranco Marchetti)....Pages 63-87
Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy (Kathryn E. White)....Pages 89-102
Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy (Daniyal J. Jafree, David A. Long, Peter J. Scambler, Dale Moulding)....Pages 103-126
Front Matter ....Pages 127-127
Transcutaneous Measurement of Glomerular Filtration Rate in Rodents (Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, Norbert Gretz)....Pages 129-137
An In Vitro Method to Analyze Glucose Uptake in Podocytes (Abigail C. Lay, Richard J. Coward)....Pages 139-143
Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro (Raina D. Ramnath, Simon C. Satchell)....Pages 145-151
Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy (Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, Berend Isermann)....Pages 153-173
Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease (Karina Barreiro, Tobias B. Huber, Harry Holthofer)....Pages 175-188
Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy (Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, Paul E. Squires)....Pages 189-201
Front Matter ....Pages 203-203
Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches (Laura J. Smyth, Katie Kerr, Seamus Duffy, Jill Kilner, Amy Jayne McKnight)....Pages 205-240
Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data—Association Studies (Marisa Canadas-Garre, Laura J. Smyth, Kerry Anderson, Katie Kerr, Amy Jayne McKnight)....Pages 241-275
Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients (Lucy Jade Newbury, Alexa Wonnacott, Kate Simpson, Timothy Bowen, Donald Fraser)....Pages 277-285
Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy (Justyna Siwy, Linda Ahonen, Pedro Magalhães, Maria Frantzi, Peter Rossing)....Pages 287-306
Front Matter ....Pages 307-307
Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice (Ioannis Bantounas, Edina Silajdžić, Adrian S. Woolf, Susan J. Kimber)....Pages 309-322
Generating Mutant Renal Cell Lines Using CRISPR Technologies (Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David A. Long, Dagan Jenkins)....Pages 323-340
Back Matter ....Pages 341-342

✦ Subjects

Biomedicine; Molecular Medicine; Nephrology; Diabetes