Uric acid (UA) sensor based on molecularly imprinted polymer-modified hanging mercury drop electrode was developed for sensitive and selective analysis in aqueous and blood serum samples. The uric acid-imprinted polymer was prepared from melamine and chloranil and coated directly onto the surface of a hanging mercury drop electrode, under charge-transfer interactions at þ 0.4 V (vs. Ag/AgCl), in model 303A electrode system connected with a polarographic analyzer/stripping voltammeter (PAR model 264A). The binding event of uric acid was detected in the imprinted polymer layer through differential pulse, cathodic stripping voltammetry (DPCSV) at optimized operational conditions [accumulation potential þ 0.4 V (vs. Ag/AgCl), accumulation time 120 s, pH 7.0, scan rate 10 mV s À1 , pulse amplitude 25 mV]. The limit of detection for UA was found to be 0.024 mg mL À1 (RSD ¼ 0.64%, S/ N ¼ 3). Under the optimized operational conditions, the sensor was able to differentiate between uric acid and other closely structural-related compounds and interfering substances. Ascorbic acid (AA), a major interferent in UA estimation, was not adsorbed on the surface of sensor electrode. The present sensor is, therefore, UA-selective at all concentrations of AA present in human blood serum samples. The pre ´cised and accurate quantification of UA have been made in the dilute as well as concentrated regions varying within limits 0.1 -4.0 and 9.8 -137.0 mg mL À1 , respectively.