The simultaneous determmatlon of theophylhne and paraxanthme m urine samples by the H-pomt standard additions method (HPSAM) IS described Samples are extracted with Cl, solid-phase extraction cartndges and chromatographed using a Hypersd Cl,-ODS column and a mobile phase conslstmg of acetomtnle-phos
Development of the H-point standard additions method for coupled liquid chromatography and UV-visible spectrophotometry
✍ Scribed by Pilar Campíns-Falcó; Francisco Bosch-Reig; Rosa Herráez-Hernández; Adela Sevillano-Cabeza
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 788 KB
- Volume
- 257
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
This work establishes the fundamentals of the H-pomt standard addltlons method for liquid chromatography for the sunultaneous analysis of binary mixtures Hnth overlapped chromatographlc peaks The method was compared mth the deconvolutlon method of peak suppression and the second derivative of elutlon profiles Dlfferent murtures of dmretics were satlsfactonly resolved
📜 SIMILAR VOLUMES
The coupling technique of synchronous fluorimetry and the H-point standard additions method was applied to determine cephalexin and cefadroxil with symmetric and seriously overlapping spectra simultaneously. Under the optimum conditions, the linear determination ranges of cephalexin and cefadroxil a
## Abstract A rapid and sensitive liquid chromatography/electrospray ionization tandem mass spectrometric (LC/ESI‐MS/MS) method has been developed to determine 1, 2‐[bis(1,2‐benzisoselenazolone‐3(2H)‐ketone)]‐ethane (BBSKE), a novel antineoplastic agent, in rat plasma. The analytes were separated o