Development of long PCR techniques to analyze deletion mutations of the human hprt gene
β Scribed by Bennett Van Houten; Yiming Chen; Janice A. Nicklas; Irene R. Rainville; J. Patrick O'Neill
- Book ID
- 114072311
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 100 KB
- Volume
- 403
- Category
- Article
- ISSN
- 0027-5107
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Deletion and insertion mutations have been found to be a major component of the in vivo somatic mutation spectrum in the hypoxanthine phosphoribosyltransferase (hprt) gene of T-lymphocytes. In a population of 172 healthy people (average age, 34; mutant frequency, 10.3 x 10(-6)), deletion/insertion m
The current study comprises the analysis of muta-assay with genomic DNA. Missense mutations were tions in 10 individuals accidentally exposed to ce-the most frequent event recovered, comprising 40% sium-137 during the 1987 radiological accident in (23/57) of the spectral sample. An excess of events