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Development of fluoroapatite chromatography for the purification of monoclonal antibody

✍ Scribed by Paul K. Ng; Tomohiko Yoshitake; Mark A. Snyder

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
150 KB
Volume
33
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Monoclonal antibody was purified from tissue culture fluid using ceramic fluoroapatite chromatography. Efficiency of the capture step was shown to be sensitive to pH and phosphate concentration. More than 90% of host cell proteins were removed by ceramic fluoroapatite chromatography. Studies regarding pH control and metal adsorption are presented.

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