Fibroblasts cultured from affected skin areas of five patients with cutaneous scleroderma were found to produce increased amounts of collagen when compared with nonaffected control cells. Total RNA was isolated from the cultures and analyzed for its level of proal(1)collagen mRNA by hybridization of
Development of cell-based immunoassays to measure type I collagen in cultured fibroblasts
โ Scribed by Brian Jones; Christine Bucks; Patrick Wilkinson; Michael Pratta; Francis Farrell; Pitchumani Sivakumar
- Book ID
- 116307968
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 986 KB
- Volume
- 42
- Category
- Article
- ISSN
- 1357-2725
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Fibroblasts are heterogeneous mesenchymal cells that play important roles in the production and maintenance of extracellular matrix. Although their heterogeneity is recognized, progenitor progeny relationships among fibroblasts and the factors that control fibroblast differentiation are poorly defin