Cell cycle analysis of human dermal fibroblasts cultured on or in hydrated type I collagen lattices

✍ Scribed by T. Kono; T. Tanii; M. Furukawa; N. Mizuno; J. Kitajima; M. Ishii; T. Hamada; K. Yoshizato

• Publisher: Springer-Verlag
• Year: 1990
• Tongue: English
• Weight: 539 KB
• Volume: 282
• Category: Article
• ISSN: 0340-3696
• DOI: 10.1007/bf00371646

✦ Synopsis

The proliferation and cell cycle phase composition of human dermal fibroblasts cultured on or in type I collagen lattices (reconstituted dermis model) were examined. On collagen lattices, as compared with conventional cultures on plastic dishes, the proliferation of human dermal fibroblasts was suppressed, being arrested at about onehalf the saturation density after 10 days of culture. In collagen lattices, proliferation was further suppressed, being nearly arrested within 4-7 days of culture. Cells were analyzed for cell cycle phases by two-color flow cytometry using DNA staining and S phase cell staining with FITCconjugated antibromodeoxyuridine antibody. After 5 days of culture, the number of S phase cells on collagen lattices was 49.3% of that on plastic dishes, with an increase in GoG 1 phase cells of 79.8%. In collagen lattices, the number of S phase cells was very small (4.3% of all cells), and most of the cells accumulated in GoG~ phase. These findings suggest that the cell cycle of fibroblasts is arrested at GoG~ phase by their interaction with collagen. On the basis of these results, the reconstituted dermis model using collagen lattice is considered to be analogous to the dermis in vivo with respect to cell growth and cell cycle phase composition.