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Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable β-glycosidases

✍ Scribed by Inge Petzelbauer; Bernd Nidetzky; Dietmar Haltrich; Klaus D. Kulbe

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
31 KB
Volume
65
Category
Article
ISSN
0006-3592

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✦ Synopsis

Figure 2. Activation and inhibition of Ss␤Gly and CelB by D-glucose.

The activity was determined in a 50 mM sodium phosphate buffer, pH 6.5, at 80°C in a 15-min long reaction, at varying concentrations of D-glucose and oNPG (᭺ Ss␤Gly, 12.5 mM oNPG; ᭹ Ss␤Gly, 22.5 mM oNPG; ᮀ CelB, 12.5 mM oNPG; CelB, 22.5 mM oNPG). V oNP,0 is the velocity of the release of oNP in the absence of added D-glucose.

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