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Development of an optical flow-through biosensor for the determination of sulphite in environmental samples

✍ Scribed by M.D.Luque de Castro; J.M. Fernández-Romero

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
622 KB
Volume
311
Category
Article
ISSN
0003-2670

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✦ Synopsis

A continuous-flow biosensor arrangement based on the dual immobilization of a biocatalyst on controlled-pore glass and the reaction product on a resin support both packed in the flow-cell of a photometric detector for the determination of sulphite is proposed. The method thus developed affords 1o.w detection and quantitation limits (3 and 10 ng ml-' of sulphite, calculated as f 3a and f lOa, respectively, for n = 20), wide linear range (10-1000 ng ml-' of sulphite), good precision (R.S.D. = 2.6% for 60 ng ml-' and 0.7% for 200 ng ml-' of the analyte), and a sampling frequency of 16 h-'. The hydrodynamic assembly has been applied to the determination and recovery of the analyte in spiked, aqueous samples with excellent results.

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